A Constructive Approach on Lethal Plants for Medicinal Use

Sep 29, 2017 · In Egypt, Traditional African Medicine was used before Agrarian Revolution. In evidence of above knowledge, information concerning healthy, lethal and toxic plants has been core factor in determination of herbal drugs. Crude drug analysis is vital in ascertaining herbal drugs.

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Determination the Lethal Dose50 (LD50 ) and Study of Acute

Determination the Lethal Dose 50 (LD 50) and Study of Acute Toxicity and Histopathological Effects of Glycosides Extract of Alhagi maurorum (Aqual) in Mice Sadiyha Yasir AL-Jubory College of Veterinary medicine - AL Qadisyia Uni. Abstract This study was curried out on the extraction glycosides constituents of shoot and seeds of Alhagi

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Medicinal plants - from traditional use to toxicity assessment: a review

The lethal dose (LD50) is defined as the dosage of a substance which kills 50 per cent of the animals in a particular group, usually determined in Determination of appropriate dose is a very important issue in the study of plant extracts. Therefore, before starting the study on plants, researchers

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Determination of the lethal doses LD50 and LD90 of Euphorbia

Lethal doses (LD 50 and LD 90), were determined probit analysis (Finney, 1971). The plot probit of kill against log of doses (µL/L) provides a simple graphic representation of the dose-to- response ratio. The results of physical and chemical analyses were obtained by analysis of

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The Effect of Hydro-Alcoholic Extract of Pumpkin Seeds on

Mar 16, 2020 · 3.3. Dose Determination. Several concentrations of pumpkin seed extract were randomly selected and injected into 4 groups of 8 rats. The lethal dose (LD50) for the groups in which half of the rats died was 400 mg/kg. The maximum, average, and minimum concentrations were determined.

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PDF) Acute toxicity studies and determination of median lethal

The plant and fruit of Cirus aurantifolia has far utilized for the determination of median lethal dose; to study the toxicity pattern of the plant. The extract was found to be

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PDF Antimicrobial activity of some medicinal plant extracts

2.6 extraction techniques of plant extracts. 2.7 isolation and identification methods. 2.8 current trends in phytochemistry and Table 7.0: 50% Inhibitory Concentration of Ten Plant Extracts and Vitamin C and their Coefficient of Determination Values

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EVALUATION OF THE LETHAL DOSE OF THE METHANOL EXTRACT OF

The largest dose that did not kill any rat was noted, as well as the smallest dose that killed all the animals. Acute Toxicity Screening This study was done to determine the median lethal dose (LD 50) of the methanol extract of Rhizophora racemosa. A total of thirty-six Albino rats of both sexes were used for this study.

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Total phenolic and flavonoid contents and antioxidant, anti

Sep 09, 2019 · The acute toxicity of the total ethanolic extract of Cordia myxa leaves was evaluated by measuring the lethal dose for 50% of the laboratory animals (LD 50) . Different dose levels (1, 2, 2.5, 3 up to 3.5 g/ kg, p.o) of the total ethanolic extract (suspended in 0.5% CMC) were orally administrated to different groups of mice (30 ± 5 g, each containing six mice).

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Acute Toxicity of Ethanolic Leaf Extract of Myrianthus - GRIN

Acute toxicity testing involves the determination of lethal dose, the dose that kills 50% of the tested group of animals, whereas sub-acute and chronic Sub-acute toxicity tests are employed to determine toxicity likely to arise from repeated exposures or daily dose of several weeks to several months

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Papaya Uses, Benefits & Dosage - Drugs.com Herbal Database

May 22, 2020 · Orally, high-dose extracts from the leaves may cause gastric irritation (extracts from unripe fruit have shown antiulcer activity in animal models). Ezike 2009 The results of an ulcerogenic study showed an ethanolic extract of C. papaya leaves produced gastric mucosal irritation at high doses (200 mg/kg in rats).

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What is a LD50 and LC50? : OSH Answers

LD stands for "Lethal Dose". LD 50 is the amount of a material, given all at once, which causes the death of 50% (one half) of a group of test animals. The LD 50 is one way to measure the short-term poisoning potential (acute toxicity) of a material.

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PDF TOXICITY

Extraction Procedure The fresh leaves of Moringa oleifera plant were plucked and dried under room The percentage yield of the extract was determined by weighing the coarse Moringa oleifera leaf Determination of Lethal Dose 50 (LD50) Acute toxicity studies (LD50) was measured using

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Acute Toxicity Study and Determination of Median Lethal Dose of

Acute Toxicity Study and Determination of Median Lethal Dose of Catharanthus roseus in Wistar Albino therefore, the LD 50 of the plant extract is slightly higher than 5000 mg kg –1 which can lead to mortality. It is recommended that studies

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Effect of methanol extract of Synsepalum dulcificum pulp on

Acute toxicity studies and lethal dose (LD 50) test Acute toxicity studies of the methanol extract of S. dulcificum pulp was carried out by the Lorke (1983) method. A total of twenty-two conducted in two phases. In phase I, three groups of nine (9) mice per group were administered one dose of the extract daily using the

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THERAPEUTIC EFFECT OF TAMARINDUS INDICA EXTRACTS ON THE

Determination of Lethal Dose (LD 50): LD 50 of the plant extract was determined by the method 7 using thirty-three rats (Rattus norvegicus) weighing between 200-220g. The first phase, 15 rats were divided into five groups (each group contains three rats) treated with aqueous extract at doses of 125, 250, 500, 750 and 1000 mg/kg body weight.

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Dosage Determination of Ethnopharmacologically Used Fermented

Nov 15, 2020 · The result of acute toxicity showed the median lethal dose (LD 50) of the extract was 3808 mg/kg body weight of experimental animals, indicating the safety of the leaves of the plant . The result of the Widal test also revealed the activity of the extract against Salmonella in vivo ( Table 6 ).

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Sub-lethal assessment of aqueous dried leaf extract of catharanthus roseus (Linn

Lethal dose (LD 50) study . A standard method was adopted for the determination of median lethal dose (LD 50) of extract. 16 The aqueous dried leaf extract of C. roseus was given to various groups . The animals were then observed for behavioral changes and mortality for 24 hours. Sub-lethal

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Determination of LC50 and LD50 of Toxicant

2021. 2. 2. · [Dose-50% mortality time curve (p. 496)]. Determination of LD 50:. Albino Mice (Test Animal): Four batches of healthy albino mice, each consists of six individuals of almost same age group and weight are taken. The toxicant may be used in solid/liquid state.

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DETERMINATION OF LETHAL DOSE AND EFFECT OF GAMMA RAYS ON

Determination of Lethal Dose 50 (LD 50) The LD 50 was determined using Microsoft Excel 2010 based on the number of survival plants at the different doses of gamma radiation. A regression equation was used to determine the LD 50. Data were recorded for a percentage of germination at 10 day after incubation (DAI).

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Determination of Lethal Doses of Volatile and Fixed Oils of Several Plants

2012. 10. 22. · extract and results were evaluated with the method of probit analysis. Results: Maximum volume of oil administered to mice was kept below 0.5 ml. The oils of Sesamum indicum and Urtica pilulifera was completely non-lethal even at doses reaching 12.8 ml/kg and considered non-toxic. Conclusion: Lethal doses were determined for all other plants

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Obtaining the Minimum Lethal Dose against Fasciola hepatica in Vitro Using Plant

2014. 6. 19. · The plant parts (leaves, flowers and stems) were extracted with a hexane solvent using a rota-evaporator. Then 3 distillations were performed every 3 - 4 days depending on the plant. The extracts were concentrated in different vials for later evaluation. 2.2. In Vitro Fasciolicide Evaluation of the Minimum Lethal Dose with Hexane Extracts

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PDF) ACUTE TOXICITY (LETHAL DOSE 50 CALCULATION) AND

LD50=LD100-∑(a x b)/nn= total number of animal in group a= the difference between two successive doses of administered extract/substance b= the average number of dead animals in two successive doses.LD100= Lethal dose causing the 100% death of all test animals.Hodge and Sterner scale (Table 2b) Histopathology studyImmediately after death of

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Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug

Jan 01, 2015 · arithmetic method of Karber [15] was used for the determination of LD50. LD50 LD100 = −∑ ×(a bn) n = total number of animal in a group. a = the difference between two successive doses of administered extract/substance. b = the average number of dead animals in two successive doses. LD100 = Lethal dose causing the 100% death of all test

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Determination of Lethal Dose (LD5... preview & related info | Mendeley

LD50 (50% Lethal Dose) of venom is the prime parameter to determine the toxicity and lethality of venom extracted from the four poisonous snakes present in the Pakistan. The main objective of this study waste produces highly potent and cost- effective anti-snake venom serum by the determination

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